LOCUS       40924_49222        5653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD048, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    29..34
                     /label=ZraI
                     /note="ZraI"
     misc_feature    32..3844
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    32..52
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    53..58
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(257..730)
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /note="repB-pANS; #1 amino acid V to M; similar to INSD 
                     accession AAB21874; Synechococcus elongatus PCC7942"
                     /protein_id="AII99712.1"
                     /translation="MGSCIAPQVRYLHGLKVEELLQRRDWFSVDDPLIQEIVTTATSHR
                     ASAAQILKIQITDKTRASTIVRALLRMIGFKLKARQKRIAGKSTWIYQVVPNEAIAELP
                     LEAIEAHWLENARSSQGLGSCHEIPNRRSKPITAHFRDTASGQVSTTSTETAA"
     CDS             complement(685..3315)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-pANS; similar to INSD accession AAB21875; 
                     Synechococcus elongatus PCC7942"
                     /protein_id="AII99713.1"
                     /translation="MAFIANHLQRSQIPTRCELIDQLDRLPPNWRFCVVNSRKAPFDPG
                     WQNNPLDHAAVAERIRSDRRVTGIGLLTGPASGGLIAVDFDGPTAHEALPEGLTLTELP
                     PTVAYTSGKPGRHQRLYQVPQERWAAIATQKLHSPDGDLLELRWNKLQSVIVGQHPETG
                     AYRWVEGCAPWEIEVAEAPPELLDAMERQDRQTAPKSYKPIVSAPASDDEVAIARTMLA
                     HVPASYADDYESWVAVGMALQSVSDALLDDWIAWSAQSSKFDGNRKLERKWASFKGSGI
                     TIATLAKLAKEGGYRPPKLTRQEQREYQREVANEWQRLTRSPNRLHTGGYINGDYIPQP
                     EQHRLVCLNAAMGTGKTEAIAGHLQPLIGTGIPVVLITHRRTLGAALGKRLGVPWAEEC
                     VPGSDLRMIGLGGCLDSMHPASKLRFSAHGWQRAIVVIDEVELWAEHLLSGKTEIAKHR
                     PEVMAEIAGLLAGGRQVIAADAMLSDVSIALLEALTGSTAYLVSSTVQPFSGAPVYQVG
                     SDRELMAMAVEMVQAGKRLAVFCDRQKLSQSRPSLYSAATIAQQLSEACGQPVPVIDSE
                     ANKRGALETLKLNPQMFINCCPHLVFTSVIDAGLSITQGVGDRPFDAVLIFAGAGHIAP
                     GAIAQIAGRVRSNVPRFITCPDTSNLMRAGNGSIDPTEVADGLKRHQQVILAQLSQAAI
                     KIGEPVASQAYLTAWAELAARRNRDGLTYAATVEQLLRLNGFTWQDWDGAIDPFLQEVV
                     DELQRETAEALADASAEAILSSPDLTPKEAEAIASADFANAEQLAALARHRCQQRWGDC
                     TRELLAADDDGLYAPLRLLFWLSLGKDAVPLAMPPSSTNGAIARCSDPILWAAVSRPKC
                     GTCMG"
     misc_feature    3818..3823
                     /label=SacI
                     /note="SacI"
     misc_feature    3824..3844
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons; synthetic"
     misc_feature    3842..3847
                     /label=ZraI
                     /note="ZraI"
     rep_origin      complement(3929..4517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4691..5548)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5549..5653)
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacga cgtcgggggc ccccgggggg attctagacc
       61 gagctgggca ctgacagcct cagtgagcgg ctctcggaag cgctcgagca ttggccacat
      121 gtgcttgggt gctgcgatgg ccagcgttcc actatcgaag ctgtaggcgc ggcacggtta
      181 gccgcaaacc atcgcaagcc ttcgttctcg atcgcggcga tcgcagttgt ccaaagctga
      241 tcgagttcgg cttgtgtcat gctgcggtct ccgtagatgt agtgctgacc tgacctgacg
      301 cggtgtcacg aaaatgcgca gtgattggtt ttgatcttct attagggatt tcgtgacagc
      361 tccccagtcc ctgactagat cgggcgtttt caagccaatg agcttcgatc gcctcaagcg
      421 gcagttcagc aatcgcctca ttcggcacga cttggtagat ccaagtggac ttgcccgcga
      481 tgcgcttctg ccgtgccttg agcttgaagc cgatcatccg cagcagcgcc cgcacgatgg
      541 tgctggctct ggtcttgtcg gtgatctgaa tcttgaggat ctgtgcggca ctagcccggt
      601 ggcttgtggc ggtcgtgacg atctcttgaa tcagcggatc atcaacgctg aaccagtcgc
      661 ggcgttgcag caactcttcc accttcagcc catgcaggta ccgcacttgg ggcgcgatac
      721 agctgcccac aagatcgggt ccgaacatcg ggcgatcgcg ccgtttgtcg agcttggcgg
      781 catcgcgagc ggcacggcat ccttgccaag gctgagccag aacagcagtc ttagcggggc
      841 gtatagaccg tcgtcatcag cggctagtag ttcacgggtg cagtctcccc agcgttgctg
      901 gcagcggtgc cgagcaaggg cagccaactg ttccgcgtta gcgaagtccg cagacgcgat
      961 cgcctcagct tccttcgggg tcagatcagg tgaactcagg atcgcctcgg ctgaggcatc
     1021 agccagcgct tcagcagtct cccgttgcag ttcgtcaacg acctcttgaa gaaagggatc
     1081 gatcgcgccg tcccagtctt gccaagtgaa cccgttgagc cgtagaagct gctccaccgt
     1141 ggcggcatag gtcaacccgt cacggttgcg acgggcggcc aattctgccc atgcagtcaa
     1201 gtaggcttgc gatgcgaccg gctcgccgat cttgatcgcc gcctgtgaca gctgagccaa
     1261 gatcacttgt tggtgccgct tcaggccgtc ggcaacctcg gttgggtcga tgctaccgtt
     1321 gccagctcgc atcaaattgg acgtgtcggg gcaggtgatg aatcggggga cgttgctccg
     1381 cacccgtcct gcaatctgcg cgatcgcacc tggggcgatg tgcccagctc cggcgaagat
     1441 cagcacggca tcaaatgggc gatcgcctac cccttgggtg atcgacaagc cagcatcgat
     1501 cacgctcgtg aagacaaggt gggggcaaca gttgatgaac atctgcgggt tgagcttcag
     1561 cgtttccaaa gccccccgct tattggcttc gctgtcgatg accggaaccg gctgaccgca
     1621 ggcttcggag agctgttggg cgatcgtcgc tgcggaatac agggacggcc gcgactggct
     1681 cagcttctgg cggtcgcaga agactgctaa gcgcttgccc gcctgaacca tttcgaccgc
     1741 catcgccatg agttcgcgat cgctgccaac ctgatagacc ggagcaccgc tgaagggttg
     1801 gacggtactg ctgaccagat atgcggttga tcctgtcagt gcttccaaca gggcgatcga
     1861 cacatccgac agcatcgcgt cagcggcgat aacctgccgc ccccctgcga gtagtccagc
     1921 aatttcagcc atgacttcgg ggcgatgttt agcgatctcg gtcttgccgc tcagcaggtg
     1981 ctcagcccac agttccacct catcgatgac gacgatcgcc cgttgccatc cgtgggcgct
     2041 gaagcgcagc ttgctggcgg ggtgcatgga gtctaggcaa ccgccaaggc cgatcatccg
     2101 aaggtcggaa ccgggaacgc attcttcagc ccacggcacc cctaagcgct tgcccaaggc
     2161 cgcgcccaag gtacggcgat gcgtgatcaa cacgacagga atgccggtac caatgagcgg
     2221 ctggagatgc cccgcgatcg cctctgtctt gcccgtcccc atcgcagcat tcaggcagac
     2281 aaggcgatgc tgttccggct gcgggatgta gtcgccgttg atgtacccgc cggtgtgtaa
     2341 gcggttaggg ctacgggtca gccgctgcca ctcgttcgcg acctcgcgct gatattcccg
     2401 ctgctcttgt cgagtcagct ttggcgggcg gtagccgcct tctttggcca gcttggccag
     2461 tgtggcgatc gtgatgccgc tgcccttgaa gctggcccac ttccgctcta gcttgcggtt
     2521 gccgtcgaac ttggacgatt gcgctgacca tgcaatccag tcatctaaca gggcatcgct
     2581 gactgactgc agggccatcc cgacagccac ccagctctcg tagtcgtctg cgtagctggc
     2641 aggtacatgg gccagcatgg tgcgggcgat cgcgacctca tcatcagaag ctggagcact
     2701 gacgatcggc ttataggatt tcggcgcggt ctgccggtct tgccgctcca ttgcatccag
     2761 caattcgggc ggggcctctg caacctcgat ctcccaaggt gcgcagcctt ccacccagcg
     2821 atatgcgccg gtctcagggt gttgaccaac gatgactgat tgcagcttgt tccaacgcag
     2881 ctctaacaga tcaccgtctg ggctgtgaag cttctgcgtg gcgatcgcag cccatcgctc
     2941 ttggggtacc tgatagagcc gttggtggcg tcctggcttg ccagatgtat atgcaactgt
     3001 gggcggtaat tctgtcagcg tcagcccttc tggcagtgcc tcatgagccg ttgggccatc
     3061 gaagtcaact gcgattaacc caccgcttgc ggggccagta agcagaccga ttccggtaac
     3121 gcgccgatcg ctgcggatac gttcagcaac agcagcatgg tcaagcgggt tattttgcca
     3181 gccagggtca aatggtgcct tgcgactgtt aacgacgcag aagcgccagt ttgggggcag
     3241 ccggtcaagt tgatcaatta attcgcagcg ggtcgggatc tgcgaccttt gcagatgatt
     3301 ggcgataaat gccatacttc tgttgagtta tttttcctga cctcccctgg gcaaagtttg
     3361 ccgacctggc ctgggggggg ttttgctttg attgccgata actatagctg gaatgaatac
     3421 ggcaggtggc aaggctgcct cgaattgatc ggcaagccag agcgcgatcg cccctggctg
     3481 tgatgagact tagacgccgg acgctgtcga gatccgaacg atcggacggt gatcgactac
     3541 gcagcagccg aagctgtgct cagccttggc gcggtaaaag cccgtctcga actgcacctc
     3601 gctagttagc tgaggcgcgg gcgtcaggcg tcccagctcc agaggtaggt tgccgcgtgc
     3661 aactaggtag tagtagctgc cgctcagccg tgcgtcgatc gcaacctcca gcgaccaagg
     3721 gttgaactca tcgcggctgt tgacttgcag atctgccacc aactgacgga taggcagctc
     3781 actctcaggc gacgcgagga tcagtccagg ctcttgagag ctcatccccg ggcccgggcc
     3841 cgacgtcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5641 taggggttcc gcg
//
